Phenotyping the quality of complex medium components by simple online-monitored shake flask experiments
نویسندگان
چکیده
منابع مشابه
Phenotyping the quality of complex medium components by simple online-monitored shake flask experiments
BACKGROUND Media containing yeast extracts and other complex raw materials are widely used for the cultivation of microorganisms. However, variations in the specific nutrient composition can occur, due to differences in the complex raw material ingredients and in the production of these components. These lot-to-lot variations can affect growth rate, product yield and product quality in laborato...
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The microbiological leaching of a sulfide ore sample was investigated in shake flask experiments. The ore sample contained pyrite, pyrrhotite, pentlandite, sphalerite, and chalcopyrite as the main sulfide minerals. The tests were performed at eight different temperatures in the range of 4 to 37 degrees C. The primary data were used for rate constant calculations, based on kinetic equations unde...
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Shake-flask cultures are widely used for screening of high producing strains. To select suitable strains for production scale, cultivation parameters should be applied that provide optimal growth conditions. A novel method of measuring respiratory activity in shake-flask cultures was employed to analyze Escherichia coli fermentation under laboratory conditions. Our results suggest that the leng...
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BACKGROUND Pretreated lignocellulosic biomass is considered as a suitable feedstock for the sustainable production of chemicals. However, the recalcitrant nature of cellulose often results in very cost-intensive overall production processes. A promising concept to reduce the costs is consolidated bioprocessing, which integrates in a single step cellulase production, cellulose hydrolysis, and fe...
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Hydrometallurgical facilities processing sulfide based ores produce waste residues in the form of sludges that contain concentrations of metals, as well as metal sulfides. As a part of the waste characterization and risk assessment process, a statistical design of experiment was used to assess the significant factors and interactions in the residue leaching process. Two shake flask experiments,...
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2014
ISSN: 1475-2859
DOI: 10.1186/s12934-014-0149-5